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stempro34 sfm medium  (Thermo Fisher)


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    Structured Review

    Thermo Fisher stempro34 sfm medium
    Stempro34 Sfm Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stempro34+sfm+medium/stempro+34+sfm/pm39126918-31-10-13
    Average 90 stars, based on 1 article reviews
    stempro34 sfm medium - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Generation of an induced pluripotent stem cell line (NCHi016-A) from a 5-year-old female with pulmonary atresia with intact ventricular septum and one-and-half ventricle palliation.
    Article Snippet: .. Patient PBMCs were isolated and cultured for one week in StemPro34 SFM medium (Thermo Fisher Scientific) supplemented with 100 ng/ mL SCF (PeproTech), 20 ng/mL IL3 (PeproTech), 100 ng/mL FLT3 (Thermo Fisher Scientific), 20 ng/mL EPO (Thermo Fisher Scientific), 1X GlutaMAX (Thermo Fisher Scientific), and 20 ng/mL IL6 (Gibco). .. On day 0 (D0), PBMCs were transfected using CytoTuneTM-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific).

    Article Title: An induced pluripotent stem cell line (EHTJUi004-A) generated from a neonate with c.4683_4684delCT:p.Leu1563fs mutation in the gene DSP causing Familial Arrhythmogenic Right Ventricular Dysplasia (ARVD).
    Article Snippet: .. The UCBMCs were isolated by lymphocyte separation medium (TBDscience) and then cultured in UCBMC medium including StemPro34 SFM medium supplemented with SCF (100 ng/mL), FLT-3 (100 ng/ mL), IL-3 (20 ng/mL), IL-6 (20 ng/mL) and L-Glutamine (2 mM) (Thermo Fisher). .. After four days culture, the infection was executed by using Cyto TuneTM-iPS 2.1 Sendai Reprogramming Kit (Thermo Fisher, A34546) according the manufacturer’s instruction.

    Article Title: Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS).
    Article Snippet: .. Patient PBMCs were isolated and incubated for one week in StemPro34 SFM medium (Thermo Fisher Scientific) supplemented with 100 ng/ mL SCF (PeproTech), 100 ng/mL FLT3 (Thermo Fisher Scientific), 20 ng/mL IL3 (PeproTech), 20 ng/mL IL6 (Gibco), 20 ng/mL EPO (Thermo Fisher Scientific), and 1× GlutaMAX (Thermo Fisher Scientific). .. PBMCs were then transfected using the CytoTuneTM-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.

    Cell Culture:

    Article Title: Generation of an induced pluripotent stem cell line (NCHi016-A) from a 5-year-old female with pulmonary atresia with intact ventricular septum and one-and-half ventricle palliation.
    Article Snippet: .. Patient PBMCs were isolated and cultured for one week in StemPro34 SFM medium (Thermo Fisher Scientific) supplemented with 100 ng/ mL SCF (PeproTech), 20 ng/mL IL3 (PeproTech), 100 ng/mL FLT3 (Thermo Fisher Scientific), 20 ng/mL EPO (Thermo Fisher Scientific), 1X GlutaMAX (Thermo Fisher Scientific), and 20 ng/mL IL6 (Gibco). .. On day 0 (D0), PBMCs were transfected using CytoTuneTM-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific).

    Article Title: An induced pluripotent stem cell line (EHTJUi004-A) generated from a neonate with c.4683_4684delCT:p.Leu1563fs mutation in the gene DSP causing Familial Arrhythmogenic Right Ventricular Dysplasia (ARVD).
    Article Snippet: .. The UCBMCs were isolated by lymphocyte separation medium (TBDscience) and then cultured in UCBMC medium including StemPro34 SFM medium supplemented with SCF (100 ng/mL), FLT-3 (100 ng/ mL), IL-3 (20 ng/mL), IL-6 (20 ng/mL) and L-Glutamine (2 mM) (Thermo Fisher). .. After four days culture, the infection was executed by using Cyto TuneTM-iPS 2.1 Sendai Reprogramming Kit (Thermo Fisher, A34546) according the manufacturer’s instruction.

    Incubation:

    Article Title: Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS).
    Article Snippet: .. Patient PBMCs were isolated and incubated for one week in StemPro34 SFM medium (Thermo Fisher Scientific) supplemented with 100 ng/ mL SCF (PeproTech), 100 ng/mL FLT3 (Thermo Fisher Scientific), 20 ng/mL IL3 (PeproTech), 20 ng/mL IL6 (Gibco), 20 ng/mL EPO (Thermo Fisher Scientific), and 1× GlutaMAX (Thermo Fisher Scientific). .. PBMCs were then transfected using the CytoTuneTM-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.

    Single Cell:

    Article Title: Integrated transcriptomic and regulatory network analyses identify microRNA-200c as a novel repressor of human pluripotent stem cell-derived cardiomyocyte differentiation and maturation.
    Article Snippet: Human ESC cultivation and differentiation: The HES2 (ESI) hESC cell line was cultured using a mTeSRTM1 feeder-free culture system (StemCell Technologies, Vancouver, BC, Canada).18 Undifferentiated hESCs were seeded onto Matrigel-coated plates at 3 105 cells/10 cm2 in mTeSR1 medium for 4 days until they were 80%–90% confluent. .. To initiate cardiac differentiation (Day 0), single-cell suspensions of hESCs were placed in ultralow-attachment six-well plates (Corning, NY, USA) and was performed as previously described.18 On Day 8, cells were transferred to a normoxic environment and maintained in StemPro34 SFM medium (Thermo Fisher, Waltham, MA, USA) with ascorbic acid (50mg/ml; Sigma-Aldrich, St. Louis, MO, USA). .. For confirmatory experiments, H7 hESCs were maintained in E8 media (Thermo Fisher).

    CMC:

    Article Title: Inhibition of Allergic Reactivity through Targeting FcεRI-Bound IgE with Humanized Low-Affinity Antibodies.
    Article Snippet: PBMC (2 3 109) were used to isolate the human CD34+ blood hematopoietic stem cells using Easysep Human CD34 Positive Selection Kit (Stemcell Technologies) per manufacturer’s instructions. .. CMC were developed over 8 wk in StemPro34 SFM medium (Life Technologies) in the presence of SCF and IL-6, plus IL-3 in the first week only. ..



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